Review



egfr kinase assay kit  (BPS Bioscience)


Bioz Verified Symbol BPS Bioscience is a verified supplier
Bioz Manufacturer Symbol BPS Bioscience manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    BPS Bioscience egfr kinase assay kit
    Egfr Kinase Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfr+kinase+assay+kit/EGFR+Kinase+Assay+Kit/pm42790246-477-8-15
    Average 95 stars, based on 127 article reviews
    egfr kinase assay kit - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Activity Assay:

    Article Title: High-throughput screening of EGFR/Ca 2+ signaling modulators in cardiac hypertrophy using a tetrahedral DNA nanostructure-based hESC platform
    Article Snippet: For drug screening, hESC-CMs were pretreated with natural compounds (10 μM) for 3 h, followed by stimulation with Ang II (10 μM) for 24 h. TDN-EA (10 nM) was added, and fluorescence was measured using a multimode plate reader (PerkinElmer; Boston, MA, USA). .. Cellular EGFR activity was quantified using an EGFR Kinase Assay Kit (BPS Bioscience, San Diego, CA, USA), following the manufacturer's protocol. .. Luminescence was recorded using a multimode plate reader (PerkinElmer; Boston, MA, USA).

    Article Title: High-Throughput Screening of EGFR/Ca2+ Signaling Modulators in Cardiac Hypertrophy Using a Tetrahedral DNA Nanostructure-Based hESC Platform
    Article Snippet: .. Cellular EGFR activity was quantified using an EGFR Kinase Assay Kit (BPS Bioscience, San Diego, CA, USA), following the manufacturer’s protocol. ..

    Kinase Assay:

    Article Title: High-throughput screening of EGFR/Ca 2+ signaling modulators in cardiac hypertrophy using a tetrahedral DNA nanostructure-based hESC platform
    Article Snippet: For drug screening, hESC-CMs were pretreated with natural compounds (10 μM) for 3 h, followed by stimulation with Ang II (10 μM) for 24 h. TDN-EA (10 nM) was added, and fluorescence was measured using a multimode plate reader (PerkinElmer; Boston, MA, USA). .. Cellular EGFR activity was quantified using an EGFR Kinase Assay Kit (BPS Bioscience, San Diego, CA, USA), following the manufacturer's protocol. .. Luminescence was recorded using a multimode plate reader (PerkinElmer; Boston, MA, USA).

    Article Title: Cell membrane electrostatically cloaked dendrimeric nanocarbons for high-bioactivity and -stability natural drug screening.
    Article Snippet: Methanol (99.8%, HPLC grade, 34860), acetonitrile (99.9%, HPLC grade, 34851), formic acid (98%, HPLC grade, 43804) and alamethicin (≥98%, Sigma-Aldrich, A4665) were purchased from Merck (Darmstadt, Germany). .. EGFR Kinase Assay Kit (40324) was purchased from BPS Bioscience and KinaseGlo MAX Luminescent Kinase Assay (V6071) was from Promega. .. Multimode Microplate Reader (Synergy H1, BioTek) and Solid White 96-well Plates (#3912) is from Corning.

    Article Title: Synthesis of Imidazole-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine-Linked 1,2,3-Triazole Hybrids as Potent EGFR-Targeting Anti-Lung Cancer Agents
    Article Snippet: Objective: This study describes the design, synthesis, and biological evaluation of novel imidazole-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine-1,2,3-triazole hybrids acting as potent inhibitors of the epidermal growth factor receptor (EGFR) in lung cancer pathways.. Methods: A series of 1,2,3-triazole derivatives (VIIa–VIIo) was synthesized via a one-pot 1,3-dipolar cycloaddition reaction of 6-(chloromethyl)-3-(1-methyl-1H-imidazol2-yl)-7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine 8,8-dioxide (V) with sodium azide and various aryl alkynes at 60°C.. The in vitro antiproliferative activity of these newly developed hybrids was evaluated against human lung cancer cell lines using the standard MTT cell viability assay.

    Article Title: Unveiling the bioactive landscape of drug inactive ingredients (DIGs) using deep transfer learning
    Article Snippet: .. ADP-Glo kinase assay kit (Promega biotech, USA); ATP (Promega biotech, USA); ß-carotene (HY-N0411, 7235-40-7, MedChemExpress, China); bicinchoninic acid assay (BCA) (Beyotime, China); chlorhexidine (T1000, 55-56-1, Targetmol, USA); dodecyl gallate (T20648, 1166-52-5, Targetmol, USA); Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) (Gibco, USA); EGFR kinase assay kit (BPS bioscience, USA); Hanks’ balanced salt solution (HBSS) (Biosharp, China); kinase-Glo luminescent kinase assay (Promega biotech, USA); 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP + ) (Aldrich, China); linoleic acid (HY-N0729, 60-33- 3, MedChemExpress, China); penicillin/streptomycin (New cell & molecular biotech, China); sodium dodecyl sulfate (SDS) (Sinopharm chemical reagent, China); SPHK1 (Carna biosciences, Japan); D-sphingosine (Aldrich, China). .. The activity of SPHK1 was measured according to the protocol established by Wuxi AppTec.

    Article Title: High-Throughput Screening of EGFR/Ca2+ Signaling Modulators in Cardiac Hypertrophy Using a Tetrahedral DNA Nanostructure-Based hESC Platform
    Article Snippet: .. Cellular EGFR activity was quantified using an EGFR Kinase Assay Kit (BPS Bioscience, San Diego, CA, USA), following the manufacturer’s protocol. ..

    Article Title: Design and Synthesis of New Quinazoline Hybrid Molecules as EGFR Targeting Antibreast Cancer Agents and Computational Studies
    Article Snippet: .. The in vitro EGFR tyrosine kinase inhibition of potent compounds was evaluated using EGFR Kinase Assay Kit (BPS Bioscience, San Diego, CA, USA) by standard method[71] utilizing erlotinib as a standard. .. Molecular docking studies were performed by taking EGFR as the target protein using AutoDock tools.

    Article Title: Design, synthesis, and molecular modeling of quinoline-linked diphenylpyrazoles as multitarget EGFR, VEGFR-2, and COX-2 inhibitors with anticancer, anti-inflammatory and apoptosis-inducing properties.
    Article Snippet: .. EGFRWT and EGFRT790M kinase activities were evaluated using EGFR Kinase Assay Kit (Catalog # 40321; BPS Bioscience, USA) and EGFRT790M Kinase Assay Kit (Catalog # 40323; BPS Bioscience, USA). ..

    Article Title: Targeting EGFR With Quinazoline-4-One/Chalcone Hybrids: Design, Synthesis, and Anticancer Evaluation.
    Article Snippet: A series of novel quinazoline‐4‐one/chalcone hybrids (4a–4j) were synthesized and evaluated as epidermal growth factor receptor (EGFR) inhibitors with anticancer activity.. The target compounds were in vitro tested against MCF‐7 and HepG2 cancer cell lines, and the EGFR enzyme.. Compounds 4a and 4g were the most potent EGFR inhibitors (IC50 = 0.09 μM and 0.10 μM, respectively), compared to the standard erlotinib (IC50 = 0.16 μM).

    Synthesized:

    Article Title: Synthesis of Imidazole-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine-Linked 1,2,3-Triazole Hybrids as Potent EGFR-Targeting Anti-Lung Cancer Agents
    Article Snippet: Objective: This study describes the design, synthesis, and biological evaluation of novel imidazole-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine-1,2,3-triazole hybrids acting as potent inhibitors of the epidermal growth factor receptor (EGFR) in lung cancer pathways.. Methods: A series of 1,2,3-triazole derivatives (VIIa–VIIo) was synthesized via a one-pot 1,3-dipolar cycloaddition reaction of 6-(chloromethyl)-3-(1-methyl-1H-imidazol2-yl)-7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine 8,8-dioxide (V) with sodium azide and various aryl alkynes at 60°C.. The in vitro antiproliferative activity of these newly developed hybrids was evaluated against human lung cancer cell lines using the standard MTT cell viability assay.

    Article Title: Targeting EGFR With Quinazoline-4-One/Chalcone Hybrids: Design, Synthesis, and Anticancer Evaluation.
    Article Snippet: A series of novel quinazoline‐4‐one/chalcone hybrids (4a–4j) were synthesized and evaluated as epidermal growth factor receptor (EGFR) inhibitors with anticancer activity.. The target compounds were in vitro tested against MCF‐7 and HepG2 cancer cell lines, and the EGFR enzyme.. Compounds 4a and 4g were the most potent EGFR inhibitors (IC50 = 0.09 μM and 0.10 μM, respectively), compared to the standard erlotinib (IC50 = 0.16 μM).

    Isolation:

    Article Title: Synthesis of Imidazole-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine-Linked 1,2,3-Triazole Hybrids as Potent EGFR-Targeting Anti-Lung Cancer Agents
    Article Snippet: Objective: This study describes the design, synthesis, and biological evaluation of novel imidazole-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine-1,2,3-triazole hybrids acting as potent inhibitors of the epidermal growth factor receptor (EGFR) in lung cancer pathways.. Methods: A series of 1,2,3-triazole derivatives (VIIa–VIIo) was synthesized via a one-pot 1,3-dipolar cycloaddition reaction of 6-(chloromethyl)-3-(1-methyl-1H-imidazol2-yl)-7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine 8,8-dioxide (V) with sodium azide and various aryl alkynes at 60°C.. The in vitro antiproliferative activity of these newly developed hybrids was evaluated against human lung cancer cell lines using the standard MTT cell viability assay.

    Acid Assay:

    Article Title: Unveiling the bioactive landscape of drug inactive ingredients (DIGs) using deep transfer learning
    Article Snippet: .. ADP-Glo kinase assay kit (Promega biotech, USA); ATP (Promega biotech, USA); ß-carotene (HY-N0411, 7235-40-7, MedChemExpress, China); bicinchoninic acid assay (BCA) (Beyotime, China); chlorhexidine (T1000, 55-56-1, Targetmol, USA); dodecyl gallate (T20648, 1166-52-5, Targetmol, USA); Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) (Gibco, USA); EGFR kinase assay kit (BPS bioscience, USA); Hanks’ balanced salt solution (HBSS) (Biosharp, China); kinase-Glo luminescent kinase assay (Promega biotech, USA); 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP + ) (Aldrich, China); linoleic acid (HY-N0729, 60-33- 3, MedChemExpress, China); penicillin/streptomycin (New cell & molecular biotech, China); sodium dodecyl sulfate (SDS) (Sinopharm chemical reagent, China); SPHK1 (Carna biosciences, Japan); D-sphingosine (Aldrich, China). .. The activity of SPHK1 was measured according to the protocol established by Wuxi AppTec.

    Modification:

    Article Title: Unveiling the bioactive landscape of drug inactive ingredients (DIGs) using deep transfer learning
    Article Snippet: .. ADP-Glo kinase assay kit (Promega biotech, USA); ATP (Promega biotech, USA); ß-carotene (HY-N0411, 7235-40-7, MedChemExpress, China); bicinchoninic acid assay (BCA) (Beyotime, China); chlorhexidine (T1000, 55-56-1, Targetmol, USA); dodecyl gallate (T20648, 1166-52-5, Targetmol, USA); Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) (Gibco, USA); EGFR kinase assay kit (BPS bioscience, USA); Hanks’ balanced salt solution (HBSS) (Biosharp, China); kinase-Glo luminescent kinase assay (Promega biotech, USA); 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP + ) (Aldrich, China); linoleic acid (HY-N0729, 60-33- 3, MedChemExpress, China); penicillin/streptomycin (New cell & molecular biotech, China); sodium dodecyl sulfate (SDS) (Sinopharm chemical reagent, China); SPHK1 (Carna biosciences, Japan); D-sphingosine (Aldrich, China). .. The activity of SPHK1 was measured according to the protocol established by Wuxi AppTec.

    In Vitro:

    Article Title: Design and Synthesis of New Quinazoline Hybrid Molecules as EGFR Targeting Antibreast Cancer Agents and Computational Studies
    Article Snippet: .. The in vitro EGFR tyrosine kinase inhibition of potent compounds was evaluated using EGFR Kinase Assay Kit (BPS Bioscience, San Diego, CA, USA) by standard method[71] utilizing erlotinib as a standard. .. Molecular docking studies were performed by taking EGFR as the target protein using AutoDock tools.

    Inhibition:

    Article Title: Design and Synthesis of New Quinazoline Hybrid Molecules as EGFR Targeting Antibreast Cancer Agents and Computational Studies
    Article Snippet: .. The in vitro EGFR tyrosine kinase inhibition of potent compounds was evaluated using EGFR Kinase Assay Kit (BPS Bioscience, San Diego, CA, USA) by standard method[71] utilizing erlotinib as a standard. .. Molecular docking studies were performed by taking EGFR as the target protein using AutoDock tools.

    Mutagenesis:

    Article Title: Targeting EGFR With Quinazoline-4-One/Chalcone Hybrids: Design, Synthesis, and Anticancer Evaluation.
    Article Snippet: A series of novel quinazoline‐4‐one/chalcone hybrids (4a–4j) were synthesized and evaluated as epidermal growth factor receptor (EGFR) inhibitors with anticancer activity.. The target compounds were in vitro tested against MCF‐7 and HepG2 cancer cell lines, and the EGFR enzyme.. Compounds 4a and 4g were the most potent EGFR inhibitors (IC50 = 0.09 μM and 0.10 μM, respectively), compared to the standard erlotinib (IC50 = 0.16 μM).



    Similar Products

    95
    BPS Bioscience egfr kinase assay kit
    Egfr Kinase Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfr+kinase+assay+kit/EGFR+Kinase+Assay+Kit/pm42790246-477-8-15
    Average 95 stars, based on 1 article reviews
    egfr kinase assay kit - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    94
    BPS Bioscience egfrt790m kinase assay kit
    Egfrt790m Kinase Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfr+kinase+assay+kit/EGFR(T790M)+Kinase+Assay+Kit/pm42790246-477-19-26
    Average 94 stars, based on 1 article reviews
    egfrt790m kinase assay kit - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    95
    BPS Bioscience egfr
    Dose–response curves of the <t>EGFR/VEGFR2</t> <t>inhibition</t> of compound 3f. IC 50 values were calculated using the non-linear regression dose–response curves in GraphPad prism. Values are expressed as mean ± SD of three independent trials.
    Egfr, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfr+kinase+assay+kit/EGFR+Kinase+Assay+Kit/pmc13077647-138-6-7
    Average 95 stars, based on 1 article reviews
    egfr - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    BPS Bioscience egfr inhibition assay kit
    Dose–response curves of the <t>EGFR/VEGFR2</t> <t>inhibition</t> of compound 3f. IC 50 values were calculated using the non-linear regression dose–response curves in GraphPad prism. Values are expressed as mean ± SD of three independent trials.
    Egfr Inhibition Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfr+kinase+assay+kit/EGFR+Kinase+Assay+Kit/10__25259_slash_ajc_1026_2025-143-0-4
    Average 95 stars, based on 1 article reviews
    egfr inhibition assay kit - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    BPS Bioscience egfrwt kinase assay kit
    Dose–response curves of the <t>EGFR/VEGFR2</t> <t>inhibition</t> of compound 3f. IC 50 values were calculated using the non-linear regression dose–response curves in GraphPad prism. Values are expressed as mean ± SD of three independent trials.
    Egfrwt Kinase Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfr+kinase+assay+kit/EGFR+Kinase+Assay+Kit/10__1016_slash_j__rechem__2026__103157-126-15-19
    Average 95 stars, based on 1 article reviews
    egfrwt kinase assay kit - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    BPS Bioscience egfr kinase inhibitor screening assay kit
    Dose–response curves of the <t>EGFR/VEGFR2</t> <t>inhibition</t> of compound 3f. IC 50 values were calculated using the non-linear regression dose–response curves in GraphPad prism. Values are expressed as mean ± SD of three independent trials.
    Egfr Kinase Inhibitor Screening Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfr+kinase+assay+kit/EGFR+Kinase+Assay+Kit/pmc12869285-168-5-14
    Average 95 stars, based on 1 article reviews
    egfr kinase inhibitor screening assay kit - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    Image Search Results


    Dose–response curves of the EGFR/VEGFR2 inhibition of compound 3f. IC 50 values were calculated using the non-linear regression dose–response curves in GraphPad prism. Values are expressed as mean ± SD of three independent trials.

    Journal: RSC Advances

    Article Title: Ferulic and p -coumaric acid derivatives as dual EGFR-VEGFR2 inhibitors: design, semi-synthesis, and biological investigations

    doi: 10.1039/d6ra01213b

    Figure Lengend Snippet: Dose–response curves of the EGFR/VEGFR2 inhibition of compound 3f. IC 50 values were calculated using the non-linear regression dose–response curves in GraphPad prism. Values are expressed as mean ± SD of three independent trials.

    Article Snippet: All molecules were evaluated for their EGFR (BPS Bioscience Corporation Catalog #40321) and VEGFR-2 kinase inhibition using (BPS Bioscience Corporation catalog#40325) using ELISA kit (Enzyme-Linked Immunosorbent Assay).

    Techniques: Inhibition

    Docking findings of the top-active synthesized compounds on dual kinase enzymes. Cartoon-surface 3D representation of the kinase highlighting the color-coded secondary structures; (yellow) Gly-rich loop, (red) hinge site, (magenta) activation loop, (cyan) catalytic motif, and (orange) gatekeeper residue; predicted binding modes of the 3f, 3h, and 3d compounds (blue sticks) with the redocked co-crystallized for docking protocol validation aligned co-crystallized as green sticks over the redocked blue sticked poses of EGFR's reversible inhibitor, Erlotinib (PDB ID: 4hjo) and VEGFR-2's potent reversible inhibitor, Sorafenib (PDB ID: 4asd). Only key residues of binding at a 4 Å radius around the ligand are shown as lines and polar contacts as black-dash lines.

    Journal: RSC Advances

    Article Title: Ferulic and p -coumaric acid derivatives as dual EGFR-VEGFR2 inhibitors: design, semi-synthesis, and biological investigations

    doi: 10.1039/d6ra01213b

    Figure Lengend Snippet: Docking findings of the top-active synthesized compounds on dual kinase enzymes. Cartoon-surface 3D representation of the kinase highlighting the color-coded secondary structures; (yellow) Gly-rich loop, (red) hinge site, (magenta) activation loop, (cyan) catalytic motif, and (orange) gatekeeper residue; predicted binding modes of the 3f, 3h, and 3d compounds (blue sticks) with the redocked co-crystallized for docking protocol validation aligned co-crystallized as green sticks over the redocked blue sticked poses of EGFR's reversible inhibitor, Erlotinib (PDB ID: 4hjo) and VEGFR-2's potent reversible inhibitor, Sorafenib (PDB ID: 4asd). Only key residues of binding at a 4 Å radius around the ligand are shown as lines and polar contacts as black-dash lines.

    Article Snippet: All molecules were evaluated for their EGFR (BPS Bioscience Corporation Catalog #40321) and VEGFR-2 kinase inhibition using (BPS Bioscience Corporation catalog#40325) using ELISA kit (Enzyme-Linked Immunosorbent Assay).

    Techniques: Synthesized, Activation Assay, Residue, Binding Assay, Biomarker Discovery