egfr (BPS Bioscience)
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95
Structured Review
BPS Bioscience
egfr
Egfr, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/egfr+kinase+assay+kit/EGFR+Kinase+Assay+Kit/pmc13077647-138-6-7
Average 95 stars, based on 127 article reviews
Egfr, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/egfr+kinase+assay+kit/EGFR+Kinase+Assay+Kit/pmc13077647-138-6-7
Average 95 stars, based on 127 article reviews
egfr - by Bioz Stars,
2026-10
95/100 stars
Images
1) Product Images from "Ferulic and p -coumaric acid derivatives as dual EGFR-VEGFR2 inhibitors: design, semi-synthesis, and biological investigations"
Article Title: Ferulic and p -coumaric acid derivatives as dual EGFR-VEGFR2 inhibitors: design, semi-synthesis, and biological investigations
Journal: RSC Advances
doi: 10.1039/d6ra01213b
Figure Legend Snippet: Dose–response curves of the EGFR/VEGFR2 inhibition of compound 3f. IC 50 values were calculated using the non-linear regression dose–response curves in GraphPad prism. Values are expressed as mean ± SD of three independent trials.
Techniques Used: Inhibition
Figure Legend Snippet: Docking findings of the top-active synthesized compounds on dual kinase enzymes. Cartoon-surface 3D representation of the kinase highlighting the color-coded secondary structures; (yellow) Gly-rich loop, (red) hinge site, (magenta) activation loop, (cyan) catalytic motif, and (orange) gatekeeper residue; predicted binding modes of the 3f, 3h, and 3d compounds (blue sticks) with the redocked co-crystallized for docking protocol validation aligned co-crystallized as green sticks over the redocked blue sticked poses of EGFR's reversible inhibitor, Erlotinib (PDB ID: 4hjo) and VEGFR-2's potent reversible inhibitor, Sorafenib (PDB ID: 4asd). Only key residues of binding at a 4 Å radius around the ligand are shown as lines and polar contacts as black-dash lines.
Techniques Used: Synthesized, Activation Assay, Residue, Binding Assay, Biomarker Discovery
Related Articles
Kinase Assay:Article Title: Design, synthesis and molecular modeling of new coumarin–thiazole derivatives as dual EGFR/HDAC1 inhibitors: in vitro and in vivo anticancer assays Article Snippet: Roswell Park Memorial Institute (RPMI) 1640 medium was purchased from Sigma Chem. .. The kinase activity assay of the epidermal growth factor receptor (EGFR) was performed according to the manufacturer's instructions using the Article Title: High-throughput screening of EGFR/Ca 2+ signaling modulators in cardiac hypertrophy using a tetrahedral DNA nanostructure-based hESC platform Article Snippet: For drug screening, hESC-CMs were pretreated with natural compounds (10 μM) for 3 h, followed by stimulation with Ang II (10 μM) for 24 h. TDN-EA (10 nM) was added, and fluorescence was measured using a multimode plate reader (PerkinElmer; Boston, MA, USA). .. Cellular EGFR activity was quantified using an Article Title: Scaffold-hopping strategy for pyrazolo[3,4-d]pyrimidines: In vitro and in silico studies of dual c-Met/STAT3 inhibition for enhanced antitumor activity. Article Snippet: The Vascular endothelial growth factor receptor 2 (VEGFR2) enzyme inhibitory activity was determined for pyrazolopyrimidine compound 22b using VEGFR2 kinase assay kit from BPS Bioscience, San Diego, CA (#40325) at 1 mg/ml concentration, according to the manufacturer’s instructions [95]. .. The epidermal growth factor receptor (EGFR) enzyme inhibitory activity was determined for pyrazolopyrimidine compound 22b using Article Title: Unveiling the bioactive landscape of drug inactive ingredients (DIGs) using deep transfer learning Article Snippet: .. ADP-Glo kinase assay kit (Promega biotech, USA); ATP (Promega biotech, USA); ß-carotene (HY-N0411, 7235-40-7, MedChemExpress, China); bicinchoninic acid assay (BCA) (Beyotime, China); chlorhexidine (T1000, 55-56-1, Targetmol, USA); dodecyl gallate (T20648, 1166-52-5, Targetmol, USA); Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) (Gibco, USA); Article Title: High-Throughput Screening of EGFR/Ca2+ Signaling Modulators in Cardiac Hypertrophy Using a Tetrahedral DNA Nanostructure-Based hESC Platform Article Snippet: .. Cellular EGFR activity was quantified using an Article Title: Design and Synthesis of New Quinazoline Hybrid Molecules as EGFR Targeting Antibreast Cancer Agents and Computational Studies Article Snippet: .. The in vitro EGFR tyrosine kinase inhibition of potent compounds was evaluated using Article Title: Targeting EGFR With Quinazoline-4-One/Chalcone Hybrids: Design, Synthesis, and Anticancer Evaluation. Article Snippet: A series of novel quinazoline‐4‐one/chalcone hybrids (4a–4j) were synthesized and evaluated as epidermal growth factor receptor (EGFR) inhibitors with anticancer activity.. The target compounds were in vitro tested against MCF‐7 and HepG2 cancer cell lines, and the EGFR enzyme.. Compounds 4a and 4g were the most potent EGFR inhibitors (IC50 = 0.09 μM and 0.10 μM, respectively), compared to the standard erlotinib (IC50 = 0.16 μM). Activity Assay:Article Title: High-throughput screening of EGFR/Ca 2+ signaling modulators in cardiac hypertrophy using a tetrahedral DNA nanostructure-based hESC platform Article Snippet: For drug screening, hESC-CMs were pretreated with natural compounds (10 μM) for 3 h, followed by stimulation with Ang II (10 μM) for 24 h. TDN-EA (10 nM) was added, and fluorescence was measured using a multimode plate reader (PerkinElmer; Boston, MA, USA). .. Cellular EGFR activity was quantified using an Article Title: Scaffold-hopping strategy for pyrazolo[3,4-d]pyrimidines: In vitro and in silico studies of dual c-Met/STAT3 inhibition for enhanced antitumor activity. Article Snippet: The Vascular endothelial growth factor receptor 2 (VEGFR2) enzyme inhibitory activity was determined for pyrazolopyrimidine compound 22b using VEGFR2 kinase assay kit from BPS Bioscience, San Diego, CA (#40325) at 1 mg/ml concentration, according to the manufacturer’s instructions [95]. .. The epidermal growth factor receptor (EGFR) enzyme inhibitory activity was determined for pyrazolopyrimidine compound 22b using Article Title: High-Throughput Screening of EGFR/Ca2+ Signaling Modulators in Cardiac Hypertrophy Using a Tetrahedral DNA Nanostructure-Based hESC Platform Article Snippet: .. Cellular EGFR activity was quantified using an Concentration Assay:Article Title: Scaffold-hopping strategy for pyrazolo[3,4-d]pyrimidines: In vitro and in silico studies of dual c-Met/STAT3 inhibition for enhanced antitumor activity. Article Snippet: The Vascular endothelial growth factor receptor 2 (VEGFR2) enzyme inhibitory activity was determined for pyrazolopyrimidine compound 22b using VEGFR2 kinase assay kit from BPS Bioscience, San Diego, CA (#40325) at 1 mg/ml concentration, according to the manufacturer’s instructions [95]. .. The epidermal growth factor receptor (EGFR) enzyme inhibitory activity was determined for pyrazolopyrimidine compound 22b using Acid Assay:Article Title: Unveiling the bioactive landscape of drug inactive ingredients (DIGs) using deep transfer learning Article Snippet: .. ADP-Glo kinase assay kit (Promega biotech, USA); ATP (Promega biotech, USA); ß-carotene (HY-N0411, 7235-40-7, MedChemExpress, China); bicinchoninic acid assay (BCA) (Beyotime, China); chlorhexidine (T1000, 55-56-1, Targetmol, USA); dodecyl gallate (T20648, 1166-52-5, Targetmol, USA); Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) (Gibco, USA); Modification:Article Title: Unveiling the bioactive landscape of drug inactive ingredients (DIGs) using deep transfer learning Article Snippet: .. ADP-Glo kinase assay kit (Promega biotech, USA); ATP (Promega biotech, USA); ß-carotene (HY-N0411, 7235-40-7, MedChemExpress, China); bicinchoninic acid assay (BCA) (Beyotime, China); chlorhexidine (T1000, 55-56-1, Targetmol, USA); dodecyl gallate (T20648, 1166-52-5, Targetmol, USA); Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) (Gibco, USA); In Vitro:Article Title: Design and Synthesis of New Quinazoline Hybrid Molecules as EGFR Targeting Antibreast Cancer Agents and Computational Studies Article Snippet: .. The in vitro EGFR tyrosine kinase inhibition of potent compounds was evaluated using Inhibition:Article Title: Design and Synthesis of New Quinazoline Hybrid Molecules as EGFR Targeting Antibreast Cancer Agents and Computational Studies Article Snippet: .. The in vitro EGFR tyrosine kinase inhibition of potent compounds was evaluated using Mutagenesis:Article Title: Targeting EGFR With Quinazoline-4-One/Chalcone Hybrids: Design, Synthesis, and Anticancer Evaluation. Article Snippet: A series of novel quinazoline‐4‐one/chalcone hybrids (4a–4j) were synthesized and evaluated as epidermal growth factor receptor (EGFR) inhibitors with anticancer activity.. The target compounds were in vitro tested against MCF‐7 and HepG2 cancer cell lines, and the EGFR enzyme.. Compounds 4a and 4g were the most potent EGFR inhibitors (IC50 = 0.09 μM and 0.10 μM, respectively), compared to the standard erlotinib (IC50 = 0.16 μM). Synthesized:Article Title: Targeting EGFR With Quinazoline-4-One/Chalcone Hybrids: Design, Synthesis, and Anticancer Evaluation. Article Snippet: A series of novel quinazoline‐4‐one/chalcone hybrids (4a–4j) were synthesized and evaluated as epidermal growth factor receptor (EGFR) inhibitors with anticancer activity.. The target compounds were in vitro tested against MCF‐7 and HepG2 cancer cell lines, and the EGFR enzyme.. Compounds 4a and 4g were the most potent EGFR inhibitors (IC50 = 0.09 μM and 0.10 μM, respectively), compared to the standard erlotinib (IC50 = 0.16 μM). |
